qPCR Efficiency Calculator

Calculated from a negative standard-curve slope; 100% means doubling per cycle. Fit quality and inhibition require separate assessment.

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Result

Enter your values and run the tool to see your result.

Check the units, enter your values, and run the tool. Results follow the assumptions and method below.

Amplification efficiency100*(10^(-1/slope)-1)

Amplification factor per cycle10^(-1/slope)